aspS [2019-03-30 18:21:32]

aspartyl-tRNA synthetase

locus: BSU27550

pI: 4.65

mw: 65.80 kDa

protein length: 592 aa

gene length: 1779 bp

function: translation

product: aspartyl-tRNA synthetase

essential: yes

ec: 6.1.1.12

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

Gene

Coordinates: 2,814,743 2,816,521

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp) (according to Swiss-Prot)

Protein family

• class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

Modification

• phosphorylated on ser/ thr/ tyr [Pubmed|16493705]

Structure

• [PDB|1EQR] (from ''Escherichia coli'', 52% identity, 68% similarity) [Pubmed|10873442]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|69C7EF7E76821FF3D9E866838B24C3866CA9C189|hisS]-[gene|2B61D17950ADE0247B0CF4AE33548E15C0DDCD9B|aspS]

  regulatory mechanism

  • [regulon|T-box|T-box]: transcriptional antitermination, via tRNA controlled [SW|RNA switch], in [regulon|T-box|T-box]

  regulation

  • induced by histidine or aspartate limitation ([SW|T-box]) [Pubmed|19258532]
  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

References

• 19258532,15378759,16493705,26804570